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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
28/12/2020 |
Data da última atualização: |
28/12/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOUZA, L. N. B. de; DIAS, N. D. S. C.; SANTANA, V. de O.; SILVEIRA, L. A.; MEIRA, M. R.; SANTOS, E. S. L. dos; FALEIRO, F. G.; CERQUEIRA-SILVA, C. B. M. |
Afiliação: |
FABIO GELAPE FALEIRO, CPAC. |
Título: |
Amplification test and selection of markers analogue to resistance genes in species and commercial varieties of Passiflora spp. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Multi-Science Journal, v. 3, n. 1, 2020. |
Páginas: |
p. 61-75 |
Idioma: |
Português |
Conteúdo: |
BSTRACT. Brazil is highlighted as the largest producer and consumer of passion fruit (Passiflora) and holder of representative biodiversity. Studies dedicated to the genetic characterization of the genus in order to understand the existing diversity are important for the advancement of genetic improvement and conservation programs. The aim of this study was to select combinations of RGA (Resistance Genes Analogs) primers for commercial passion fruit species and varieties. To this end, amplification tests were performed on a PCR platform in 17 combinations of RGA primers for eight species and 12 commercial varieties of passion fruit from the Banco Ativo de Germoplasma Flor da Paixão. All combinations of primers have generated amplification products in at least one of the Passiflora samples. The number of combinations that generated amplification products varied from seven to 12 in the species and from three to eight in the varieties. Three to 12 combinations generated amplification products for the samples, emphasizing that the RGA markers were efficient in accessing genomic loci in the species and varieties of passion fruit. The variation of efficient combinations in each of the species and varieties attested to the importance of this research in the preliminary stages of genetic studies as auxiliary tools in conservation and genetic improvement programs. However, this study made it possible to identify combinations of primers to be prioritized in inter and intraspecific genetic characterizations of passion fruit MenosBSTRACT. Brazil is highlighted as the largest producer and consumer of passion fruit (Passiflora) and holder of representative biodiversity. Studies dedicated to the genetic characterization of the genus in order to understand the existing diversity are important for the advancement of genetic improvement and conservation programs. The aim of this study was to select combinations of RGA (Resistance Genes Analogs) primers for commercial passion fruit species and varieties. To this end, amplification tests were performed on a PCR platform in 17 combinations of RGA primers for eight species and 12 commercial varieties of passion fruit from the Banco Ativo de Germoplasma Flor da Paixão. All combinations of primers have generated amplification products in at least one of the Passiflora samples. The number of combinations that generated amplification products varied from seven to 12 in the species and from three to eight in the varieties. Three to 12 combinations generated amplification products for the samples, emphasizing that the RGA markers were efficient in accessing genomic loci in the species and varieties of passion fruit. The variation of efficient combinations in each of the species and varieties attested to the importance of this research in the preliminary stages of genetic studies as auxiliary tools in conservation and genetic improvement programs. However, this study made it possible to identify combinations of primers to be prioritized in inter and intraspecific gen... Mostrar Tudo |
Thesagro: |
Banco de Germoplasma; Passifloraceae; Seleção Genética. |
Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219730/1/FABIO-FALEIRO-Amplification-test-and-selection-of-markers-analog.pdf
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Marc: |
LEADER 02326naa a2200253 a 4500 001 2128776 005 2020-12-28 008 2020 bl uuuu u00u1 u #d 100 1 $aSOUZA, L. N. B. de 245 $aAmplification test and selection of markers analogue to resistance genes in species and commercial varieties of Passiflora spp.$h[electronic resource] 260 $c2020 300 $ap. 61-75 520 $aBSTRACT. Brazil is highlighted as the largest producer and consumer of passion fruit (Passiflora) and holder of representative biodiversity. Studies dedicated to the genetic characterization of the genus in order to understand the existing diversity are important for the advancement of genetic improvement and conservation programs. The aim of this study was to select combinations of RGA (Resistance Genes Analogs) primers for commercial passion fruit species and varieties. To this end, amplification tests were performed on a PCR platform in 17 combinations of RGA primers for eight species and 12 commercial varieties of passion fruit from the Banco Ativo de Germoplasma Flor da Paixão. All combinations of primers have generated amplification products in at least one of the Passiflora samples. The number of combinations that generated amplification products varied from seven to 12 in the species and from three to eight in the varieties. Three to 12 combinations generated amplification products for the samples, emphasizing that the RGA markers were efficient in accessing genomic loci in the species and varieties of passion fruit. The variation of efficient combinations in each of the species and varieties attested to the importance of this research in the preliminary stages of genetic studies as auxiliary tools in conservation and genetic improvement programs. However, this study made it possible to identify combinations of primers to be prioritized in inter and intraspecific genetic characterizations of passion fruit 650 $aBanco de Germoplasma 650 $aPassifloraceae 650 $aSeleção Genética 700 1 $aDIAS, N. D. S. C. 700 1 $aSANTANA, V. de O. 700 1 $aSILVEIRA, L. A. 700 1 $aMEIRA, M. R. 700 1 $aSANTOS, E. S. L. dos 700 1 $aFALEIRO, F. G. 700 1 $aCERQUEIRA-SILVA, C. B. M. 773 $tMulti-Science Journal$gv. 3, n. 1, 2020.
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Registro original: |
Embrapa Cerrados (CPAC) |
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Registro Completo
Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
26/11/2012 |
Data da última atualização: |
26/11/2012 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
FILHO, R. C. B.; PRASTISSOLI, D.; NAVA, D. E.; MONTE, F. G.; GUIDONI, A. L.; POLANCZYK, R. A. |
Afiliação: |
Raul C. B. Filho, Departamento de Produção Vegetal CCAUFES.; Dirceu Pratissoli, Departamento de Produção Vegetal CCAUFES.; DORI EDSON NAVA, CPACT; Fernanda G. Monte, Bolsista Embrapa Clima Temperado.; ANTONIO LOURENCO GUIDONI, CPACT; Ricardo A. Polanczyk, Departamento de Fitossanidade FCAV/UNESP. |
Título: |
Biologia de Pachycoris torridus (Hemiptera: Scutelleridae) em diferentes hospedeiros. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 24., 2012, Curitiba. SEB - 40 anos de avanços da Ciência Entomológica Brasileira. Curitiba: SEB, 2012. |
Idioma: |
Português |
Palavras-Chave: |
Integrado; Percevejo-do-pinhão-manso; Plantas hospedeiras. |
Thesagro: |
Manejo. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/70775/1/1131-1.pdf
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Marc: |
LEADER 00722nam a2200205 a 4500 001 1940493 005 2012-11-26 008 2012 bl uuuu u00u1 u #d 100 1 $aFILHO, R. C. B. 245 $aBiologia de Pachycoris torridus (Hemiptera$bScutelleridae) em diferentes hospedeiros.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 24., 2012, Curitiba. SEB - 40 anos de avanços da Ciência Entomológica Brasileira. Curitiba: SEB$c2012 650 $aManejo 653 $aIntegrado 653 $aPercevejo-do-pinhão-manso 653 $aPlantas hospedeiras 700 1 $aPRASTISSOLI, D. 700 1 $aNAVA, D. E. 700 1 $aMONTE, F. G. 700 1 $aGUIDONI, A. L. 700 1 $aPOLANCZYK, R. A.
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